Part:BBa_K1351040:Design

pBS0KPspac, an IPTG-inducible replicative expression vector for

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 8274
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal BglII site found at 5862
  Illegal BamHI site found at 1378
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 8268
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 8268
  Plasmid lacks a suffix.
  Illegal XbaI site found at 8283
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2925
  Illegal AgeI site found at 5473
  Illegal AgeI site found at 6435
  Illegal AgeI site found at 7110
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2749
  Illegal BsaI.rc site found at 4188
  Illegal BsaI.rc site found at 6704
  Illegal SapI site found at 1666
  Illegal SapI.rc site found at 5686
  

Design Notes

Inducible expression vector with RFP-MCS downstream of Pspac.

Source

This vector is derived from pDG148, and the "repaired" version of pSB_Bs0K-Pspac.

===References===